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Wikipedia

Vibratome

A vibratome is an instrument used to cut thin slices of material (although, usually thicker slices than those cut in paraffin-embedded samples using a microtome). It is similar to a microtome but uses a vibrating blade to cut through tissue. The vibration amplitude, the speed, and the angle of the blade can all be controlled. Fixed or fresh tissue pieces are embedded in low gelling temperature agarose. The resulting agarose block containing the tissue piece is then attached to a metal block and sectioned while submerged in a water or buffer bath. Individual sections are then collected with a fine brush and transferred to slides or multiwell plates for staining.

Vibratome produced by Leica Biosystems

Vibratome is not a generic name but a registered trademark of Leica Biosystems Richmond, Inc. in the USA.

  • No need to dehydrate tissues prior to embedding, thus decreased loss of cell constituents
  • No messy paraffin embedding
  • Allows for agarose embedding of tissue to provide cutting stability
  • No need to deparaffinise and rehydrate sections prior to immunostaining
  • No high temperatures or harsh chemical treatments that may lead to antigen instability
  • No special microtome blades required
  • Less chance of artifacts caused by paraffin embedding or freezing
  • Decreased tissue autofluoresecence due to avoidance of formalin-fixation and paraffin embedding
  • Less wait period from tissue sampling to time of immunolabelling
  • Allows for direct creation of free-floating sections for immunohistochemistry
  • Instead of ribbons, single sections are cut and collected which are more delicate and difficult to handle.
  • Sections are generally thicker than those obtained with paraffin methods; penetration of antibodies and other reagents may be slower and thus longer incubation times may be necessary. Also, thick sections may be difficult to image with the microscope. (However, thick sections are compatible and sometimes even desirable if using confocal microscopy.)
  • Securing vibratome sections to glass slides can be difficult or impossible, due to the thickness of the sections.

Vibratome
Vibratome Language Watch Edit A vibratome is an instrument used to cut thin slices of material although usually thicker slices than those cut in paraffin embedded samples using a microtome It is similar to a microtome but uses a vibrating blade to cut through tissue The vibration amplitude the speed and the angle of the blade can all be controlled Fixed or fresh tissue pieces are embedded in low gelling temperature agarose The resulting agarose block containing the tissue piece is then attached to a metal block and sectioned while submerged in a water or buffer bath Individual sections are then collected with a fine brush and transferred to slides or multiwell plates for staining 1 Vibratome produced by Leica Biosystems Vibratome is not a generic name but a registered trademark of Leica Biosystems Richmond Inc in the USA Advantages EditNo need to dehydrate tissues prior to embedding thus decreased loss of cell constituents No messy paraffin embedding Allows for agarose embedding of tissue to provide cutting stability No need to deparaffinise and rehydrate sections prior to immunostaining No high temperatures or harsh chemical treatments that may lead to antigen instability No special microtome blades required Less chance of artifacts caused by paraffin embedding or freezing Decreased tissue autofluoresecence due to avoidance of formalin fixation and paraffin embedding Less wait period from tissue sampling to time of immunolabelling Allows for direct creation of free floating sections for immunohistochemistryDisadvantages EditInstead of ribbons single sections are cut and collected which are more delicate and difficult to handle Sections are generally thicker than those obtained with paraffin methods penetration of antibodies and other reagents may be slower and thus longer incubation times may be necessary Also thick sections may be difficult to image with the microscope However thick sections are compatible and sometimes even desirable if using confocal microscopy Securing vibratome sections to glass slides can be difficult or impossible due to the thickness of the sections 2 References Edit Improved methods for acute brain slice preparation from adult and aging animals PDF Archived from the original on July 17 2011 Retrieved June 1 2009 http www4 ncsu edu rgfranks FRANKS 20LAB 20PROTOCOLS immuno 20histio 20chemistry robertson 20vbratmimuno doc permanent dead link Retrieved from https en wikipedia org w index php title Vibratome amp oldid 988774396, wikipedia, wiki, book,

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